Shobhan Gaddameedhi
Assistant Professor, Pharmaceutical Sciences  509-368-6570 PBS 317 Spokane https://labs.wsu.edu/gaddameedhi/

Education & Fellowships

Postdoctoral fellow, University of North Carolina School of Medicine, Chapel Hill, N.C., 2008-14, Mentor: Professor Aziz Sancar, NAS member, Nobel Laureate

Ph.D. in pharmaceutical sciences, North Dakota State University, Fargo, N.D., 2008, Mentor: Dr. Satadal Chatterjee

Master of Science in plant sciences, University of Hyderabad, Hyderabad, India, 2001

Bachelor of Science in biology and chemistry, Osmania University, Hyderabad, India, 1999

Additional Training

  • Graduate summer intern, Amgen, Calif., 2007, Mentors: Drs. Angus Sinclair and Liqin Liu
  • Graduate research assistant in biological sciences, North Dakota State University, 2002-03, Mentor: Dr. Marc D. Anderson
  • Research assistant in microbiology & cell biology, Indian Institute of Science, Bangalore, India, 2002, Mentor: Dr. Lakshmi Sita
  • Research intern, International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Hyderabad, India, 2001, Mentor: Dr. Kiran K. Sharma

Honors & Awards

  • Graduate Program Teacher of the Year Award from WSU College of Pharmacy and Pharmaceutical Sciences, 2019
  • American Society for Photobiology (ASP) New Investigator Award, 2018
  • Department of Defense (DOD)/CDMRP Career Development Award, 2018
  • Environmental Mutagenesis and Genomics Society (EMGS) Newly Independent Investigator Program Award, 2018
  • PanAmerican Society for Pigment Cell Research (PASPCR) Young Investigator Travel Award, 2018
  • Graduate Program Teacher of the Year Award from WSU College of Pharmacy, 2017
  • NIH Pathway Independent Award (K99/R00), 2013
  • UNC Postdoctoral Award for Research Excellence, 2013
  • Third place poster award from the Oliver Smithies Nobel Symposium, 2012
  • EMGS New Investigator Travel Award, 2011
  • PRACS Institute Graduate Student Research Excellence Award, 2006
  • Merit Scholarship Award for being a top ranker (All India level) on the “Master’s program entrance test” at the University of Hyderabad, 1999

Research

Every cell in the body has a circadian clock that regulates the biochemical and cellular processes such as cell cycle, DNA repair, inflammation, metabolism, and sleep-wake cycles of an organism. Studies from mice and humans suggest that ~50% of protein coding genes display circadian rhythmicity somewhere in the body at transcriptional level. Disruption of circadian rhythms can occur through environmental factors such as light at night, shift work or jet lag, and there is a strong correlation between circadian clock disruption and environmental disease outcomes including carcinogenesis. There is still, however, a lack of enough evidence at the cellular and molecular levels in humans, which the Gaddameedhi Lab addresses through the following key questions:

What are the biological mechanisms regulating environmental genotoxicity via circadian disruption in humans?

How do ‘gene-environment’ interactions mediate environmental health outcomes through the circadian clock?

What are the molecular interactions between the circadian clock and environmental factors such as solar UV-B radiation, how do these interactions impact skin disease outcomes such as autoimmune disorders, aging, and skin cancers?

What is the molecular basis of the circadian clock’s role in the improved efficacy of radiation-immuno therapy in cancers?

Answering these questions will provide a mechanistic understanding of the circadian clock’s role in ‘gene-environment’ interactions and its impact on environmental disease prevention and therapeutic efficacy.

Selected Publications

Kumar PVA, Dakup P, Sarkar S, Modasia J, Madison SM, and Gaddameedhi S* (2019): It’s About Time: Advances in Understanding the Circadian Regulation of DNA Damage and Repair in Carcinogenesis and Cancer Treatment Outcomes, Yale J. Biol. Med. 92 (2): 305-316, *Corresponding author

Skornyakov E, Gaddameedhi S, Peach GM, Sparrow AR, Satterfield BC, Shattuck NL, Layton ME, Karatsoreos I, and Van Dongen HPA (2019): Cardiac autonomic activity during simulated shift work, Ind Health 57(1):118-132

Skene D, Skornyakov E, Chowdhury N, Gajula R, Middleton B, Satterfield B, Porter K, Van Dongen HPA*, and Gaddameedhi S* (2018): Separation of circadian- and behavior-driven metabolite rhythms in humans provides a window on peripheral oscillators and metabolism, Proc Natl Acad Sci USA 115 (30): 7825-7830, *Shared-senior author

Dakup P, Porter K, Little A, Gajula R, Zhang H, Skornyakov E, Kemp MG, Van Dongen HPA, and Gaddameedhi S* (2018): The circadian clock regulates cisplatin-induced toxicity and tumor regression in melanoma mouse and human models, Oncotarget 9(18): 14524-38, *Corresponding author

Sarkar S and Gaddameedhi S* (2018): UV-B Induced Erythema in Human Skin: The Circadian Clock is Ticking, J Invest Dermatol. 138:248-251, *Corresponding author

James SM, Honn KA, Gaddameedhi S and Van Dongen HPA (2017): Shift Work: Disrupted Circadian Rhythms and Sleep Implications for Health and Well-being, Curr Sleep Medicine Rep., 3(2):104-112

Dakup P and Gaddameedhi S* (2017): Impact of the Circadian Clock on UV-Induced DNA Damage Response and Photocarcinogenesis, Photochem. Photobiol., 93:296-303, *Corresponding author

Gajula RP and Gaddameedhi S* (2015): Commentary: Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure, Front Physiol., 6:276. *Corresponding author

Manjanatha MG, Shelton SD, Chen Y, Gaddameedhi S, Howard PC, Boudreau MD (2015): Development and validation of a new transgenic hairless albino mouse as a mutational model for potential assessment of photocarcinogenicity, Mutat Res., 791: 42-52

Simpson DA, Lemonie N, Morgan DS, Gaddameedhi S, and Kaufmann WK (2015): Oncogenic BRAF (V600E) Induced Clastogenesis and UVB Hypersensitivity, Cancers, 7: 1072-1090

Gaddameedhi S*, Selby CP, Kemp MG, Ye R, and Sancar A* (2015): The Circadian Clock Controls Sunburn Apoptosis and Erythema in Mouse Skin, J. Invest. Dermatol., 135: 1119- 1127. *Shared-corresponding author

Sancar A, Lindsey-Boltz LA, Gaddameedhi S, Selby CP, Ye R, Chiou YY, Kemp MG, Hu J, Lee JH, Ozturk N (2015): Circadian Clock, Cancer, and Chemotherapy, Biochemistry, 54(2): 110-123

Ye R, Selby CP, Chiou Y, Dagliyan I, Gaddameedhi S, and Sancar A (2014): Dual modes of CLOCK:BMAL1 inhibition mediated by Cryptochrome and Period proteins in the mammalian circadian clock, Genes Dev., 28:1989-1998

Kemp MG, Gaddameedhi S, Hu J, Choi JH, and Sancar A (2014): DNA Repair Synthesis and Ligation Affect the Processing of Excised Oligonucleotides Generated by Human Nucleotide Excision Repair, J. Biol. Chem., 289 (38): 26574-26583

Choi JH, Gaddameedhi S, Hu J, Kemp MG, and Sancar A (2014): Highly specific and sensitive method for measuring nucleotide excision repair kinetics of UV photoproducts in human cells, Nucleic Acids Res., 42(4), e29

Hu J, Choi JH, Gaddameedhi S, Kemp MG, Reardon JT, and Sancar A (2013): Nucleotide Excision Repair in Human Cells: Fate of the Excised Oligonucleotide Carrying DNA Damage In Vivo. J. Biol. Chem., 288 (29): 20918-20926

Lee JH, Gaddameedhi S, Ozturk N, Ye R and Sancar A (2013): DNA damage-specific control of cell death by cryptochrome in p53 mutant Ras-transformed cells. Cancer Res., 73 (2): 785-791

Gaddameedhi S, Reardon JT, Ye R, Ozturk N and Sancar A (2012): Effect of circadian clock mutations on DNA damage response in mammalian cells. Cell Cycle, 11(18): 3481-3491

Gaddameedhi S, Selby CP, Kaufmann WK, Smart RC, and Sancar A (2011): Control of Skin Cancer by the Circadian Rhythm. Proc Natl Acad Sci USA, 108(46): 18790-18795

Gaddameedhi S and Sancar A (2011): Melanoma and DNA Damage from a Distance (Farstander Effect). Pigment Cell Melanoma Res., 24: 3-4

Gaddameedhi S, Kemp MG, Reardon JT, Shields J, Smith-Roe SL, Kaufmann WK, and Sancar A (2010): Similar Nucleotide Excision Repair Capacity in Melanocytes and Melanoma Cells. Cancer Res., 70(12):4922-4930

Ozturk N, Lee JH, Gaddameedhi S and Sancar A (2009): Loss of cryptochrome reduces cancer risk in p53 mutant mice. Proc Natl Acad Sci USA, 106 (8): 2841-2846

Gaddameedhi S and Chatterjee S (2009): Association between Unfolded Protein Response, induced by 2-deoxyglucose, and hypersensitivity to cisplatin: A mechanistic study employing molecular genomics. J. Cancer Res. Ther., 5(9): 61-66

Book Chapters

Cho K, Gajula R and Gaddameedhi S* (2016): The Cutaneous Circadian Clock as a Determinant of Environmental Vulnerability: Molecular Pathways and Therapeutic Opportunities, Skin Stress Response Pathways, Springer International Biomedical Sciences, 415-432. *Corresponding author

Chatterjee S and Gaddameedhi S (2011): In-Depth Look: anti-TNF-α Therapies, Development of Therapeutic Agents Handbook, John Wiley & Sons, 1045-1092

Funding Support

(current and past funding as a PI)

  • DOD/CDMRP Award, July 2018- June 2021
  • Melanoma Research Alliance/Team Science Award, June 2018- May 2021
  • NIH/NCI R21, June 2018- May 2020
  • NIH/NIEHS R00 Award, January 2015- December 2018
  • NIH/NIEHS K99 Award, September 2013- December 2015
  • Pilot Project Award from the UNC-CEHS, 2013- 2014
  • Pilot Project Award from the NC TraCS Institute, 2012- 2013
  • Pilot Project Grant Award from the UNC-CEHS, 2011- 2012

Invited Talks

  • EMGS Annual Meeting, Washington DC, 2019
  • Texas A&M University, College Station, TX, 2019
  • Oregon State University, Corvallis, OR, 2019
  • NIH Research Conference on Sleep and the Health of Women, Bethesda, MD, 2018
  • Penn State Skin Cancer Annual Symposium, Hershey, PA, Keynote Speaker, 2018
  • American Society for Photobiology (ASP) Biennial Meeting, Tampa, FL, 2018
  • ASP New Investigator Award Recipient Lecture, Tampa, FL, 2018
  • Smerdon/Reeves Chromatin-DNA Repair Lecture, Pullman, WA, 2017
  • Radiation Research Society (RRS) Annual Meeting, Waikoloa Village, HI, 2016
  • ASP Biennial Meeting, Tampa, FL, 2016
  • American Society for Pharmacology and Experimental Therapeutics (ASPET), San Diego, CA, 2016
  • Oregon Institute of Occupational Health Sciences, Portland, OR, invited talk, 2015
  • AACR International Conference on Frontiers in Cancer Prevention Research, New Orleans, LA, 2014
  • Fred Hutchinson Cancer Research Center, Seattle, WA, 2014
  • NIH-DNA Repair Video Conference Young Investigator talk, Chapel Hill, NC, 2012

Service

Gaddameedhi currently serves as a member for the Pharm.D. Honors program committee, the vivarium user committee, the graduate student’s thesis committee, and the graduate studies senate committee.

Teaching

Gaddameedhi currently mentors 3 PhD students, 1 post-doctoral fellow, and 1 research technologist. In addition to his responsibilities of mentoring the next generation of biomedical research scientists, his classroom teaching focuses on ‘integrated pharmacology’ 4-credit course (participating instructor) to Pharm.D. professional students, ‘fundamentals of chronopharmacology’ 3-credit graduate course (primary instructor), and ‘fundamentals of toxicology’ 3-credit graduate course (participating instructor) to Pharm.D. professional and Ph.D. graduate curriculum students

updated 10/17/2019