PhD in Pharmaceutical Analysis, NIPER, S.A.S. Nagar, Punjab, India
Master of Science (Pharm.) in Pharmaceutical Analysis, NIPER, S.A.S. Nagar, Punjab, India
Pharm. in Pharmaceutical Sciences, Dept. of Pharmaceutical Sciences, HNBGU (central university), Srinagar Garhwal, Uttarakhand, India
Diploma in Pharmacy from Government Polytechnic, Srinagar Garhwal, Uttarakhand, India
Fellowships & Additional Training
Postdoctoral fellowship: Department of Pharmaceutics, University of Washington (2011-2014)
Dr. Prasad is teaching pharmacokinetics principles and PBPK modeling in following courses.
- PHARDSCI 528
- PHARMSCI 573
- Prediction of ontogeny mediated oral absorption and hepatic and renal disposition of drugs in children using physiologically-based pharmacokinetics (PBPK) modeling
- Discovery and validation of biomarkers for non-invasive and prospective prediction of drug disposition and response
- Role of drug metabolizing enzymes and transporters in steroid homeostasis
Major expertise: Quantitative proteomics, metabolomics and PBPK modeling
Keywords: Quantitative proteomics, metabolomics, PBPK modeling, pediatric drug disposition, testosterone disposition, non-CYP enzymes
PBPK prediction of ontogeny mediated alteration in hepatic drug elimination
PRINCE (Proteomics-based Research Initiative on Non-CYP enzymes), Multiple Private Sponsors (Genentech, Merck and Gilead)
Differential tissue abundance of non-CYP enzymes in human and animal models
The plasma membrane monoamine transporter (PMAT): expression and role in mIBG disposition in neuroblastoma
Role: Co-investigator (PI: Joanne Wang)
Program on Genetic and Dietary Predictors of Drug Response in Rural and AI/AN Populations
Role: Co-Investigator (PI: Thummel)
Prenatal and Early Childhood Pathways to Health
Role: Co-Investigator (PI: Karr and Sathyanarayana)
mARC proteomics, BMS
Role of mARC protein in drug metabolism: Interindividual and differential tissue abundance and activity
- We provide collaborative support to researchers on quantitative proteomics and metabolomics
- Assistant Professor (2015-2019), Department of Pharmaceutics, University of Washington, Seattle, WA
- Director, PRINCE (Proteomics-based Research Initiative on Non-CYP enzymes) (2018-Present), Department of Pharmaceutics, University of Washington, Seattle, WA
- Co-director, UWRAPT (2016-2019), Department of Pharmaceutics, University of Washington, Seattle, WA
- Acting Assistant Professor (2014-2015), Department of Pharmaceutics, University of Washington, Seattle, WA
- Acting Instructor (2012-2014), Department of Pharmaceutics, University of Washington, Seattle, WA
- Lead Scientist-UWRAPT (2011-2014), Department of Pharmaceutics, University of Washington, Seattle, WA
- Research Associate (2006-2007), Department of Metabolism and Pharmacokinetics (MAP)-Discovery, Ranbaxy Research Laboratories
Awards and honors:
- ISSX North American New Investigator Award, International Society for the Study of Xenobiotics (ISSX), ISSX, 2018 Montreal, Canada.
- Editorial Board Member, Drug Metabolism and Disposition
- Translational and Clinical Pharmacology (TCP) Division’s Early Career Faculty Showcase ASPET, 2018, San Diego, CA
- Secretary/Treasurer, Drug Metabolism and Disposition Division, American Society for Pharmacology and Experimental Therapeutics (ASPET)
Prasad B, Achour B, Artursson P, Hop CECA, Lai Y, Smith PC, Barber J, Wisniewski JR, Spellman D, Uchida Y, Zientek MA, Unadkat JD, Rostami-Hodjegan A. Toward a Consensus on Applying Quantitative Liquid Chromatography-Tandem Mass Spectrometry Proteomics in Translational Pharmacology Research: A White Paper. Clin Pharmacol Ther. 2019 Sep;106(3):525-543.
Ladumor MK, Bhatt DK, Gaedigk A, Sharma S, Thakur A, Pearce RE, Leeder JS, Bolger MB, Singh S, Prasad B. Ontogeny of Hepatic Sulfotransferases and Prediction of Age-Dependent Fractional Contribution of Sulfation in Acetaminophen Metabolism. Drug Metab Dispos. 2019 Aug;47(8):818-831.
Li CY, Basit A, Gupta A, Gáborik Z, Kis E, Prasad B. Major glucuronide metabolites of testosterone are primarily transported by MRP2 and MRP3 in human liver, intestine and kidney. J Steroid Biochem Mol Biol. 2019 Apr 5;.
Bhatt DK, Mehrotra A, Gaedigk A, Chapa R, Basit A, Zhang H, Choudhari P, Boberg M, Pearce RE, Gaedigk R, Broeckel U, Leeder JS, Prasad B. Age- and Genotype-Dependent Variability in the Protein Abundance and Activity of Six Major Uridine Diphosphate-Glucuronosyltransferases in Human Liver. Clin Pharmacol Ther. 2019 Jan;105(1):131-141.
Xu M, Saxena N, Vrana M, Zhang H, Kumar V, Billington S, Khojasteh C, Heyward S, Unadkat JD, Prasad B. Targeted LC-MS/MS Proteomics-Based Strategy To Characterize in Vitro Models Used in Drug Metabolism and Transport Studies. Anal Chem. 2018 Oct 16;90(20):11873-11882.
Zhang H, Basit A, Busch D, Yabut K, Bhatt DK, Drozdzik M, Ostrowski M, Li A, Collins C, Oswald S, Prasad B. Quantitative characterization of UDP-glucuronosyltransferase 2B17 in human liver and intestine and its role in testosterone first-pass metabolism. Biochem Pharmacol. 2018 Oct;156:32-42.
Basit A, Amory JK, Prasad B. Effect of Dose and 5α-Reductase Inhibition on the Circulating Testosterone Metabolite Profile of Men Administered Oral Testosterone. Clin Transl Sci. 2018 Sep;11(5):513-522.
Bhatt DK, Basit A, Zhang H, Gaedigk A, Lee SB, Claw KG, Mehrotra A, Chaudhry AS, Pearce RE, Gaedigk R, Broeckel U, Thornton TA, Nickerson DA, Schuetz EG, Amory JK, Leeder JS, Prasad B. Hepatic Abundance and Activity of Androgen- and Drug-Metabolizing Enzyme UGT2B17 Are Associated with Genotype, Age, and Sex. Drug Metab Dispos. 2018 Jun;46(6):888-896.
Bhatt DK, Prasad B. Critical Issues and Optimized Practices in Quantification of Protein Abundance Level to Determine Interindividual Variability in DMET Proteins by LC-MS/MS Proteomics. Clin Pharmacol Ther. 2018 Apr;103(4):619-630.
Vrana M, Whittington D, Nautiyal V, Prasad B. Database of Optimized Proteomic Quantitative Methods for Human Drug Disposition-Related Proteins for Applications in Physiologically Based Pharmacokinetic Modeling. CPT Pharmacometrics Syst Pharmacol. 2017 Apr;6(4):267-276.
Prasad B, Gaedigk A, Vrana M, Gaedigk R, Leeder JS, Salphati L, Chu X, Xiao G, Hop C, Evers R, Gan L, Unadkat JD. Ontogeny of Hepatic Drug Transporters as Quantified by LC-MS/MS Proteomics. Clin Pharmacol Ther. 2016 Oct;100(4):362-70.
Prasad B, Unadkat JD. Optimized approaches for quantification of drug transporters in tissues and cells by MRM proteomics. AAPS J. 2014 Jul;16(4):634-48.
Bhagwat Prasad’s Bibliography https://www.ncbi.nlm.nih.gov/myncbi/bhagwat.prasad.1/bibliography/public/