Bhagwat Prasad
Associate Professor, Pharmaceutical Sciences 509-358-7739 PBS 413 Spokane https://labs.wsu.edu/prasadlab/

Education

Ph.D. in Pharmaceutical Analysis, NIPER, S.A.S. Nagar, Punjab, India

M.S. (Pharm.) in Pharmaceutical Analysis, NIPER, S.A.S. Nagar, Punjab, India

Pharm. in Pharmaceutical Sciences, Dept. of Pharmaceutical Sciences, HNBGU (central university), Srinagar Garhwal, Uttarakhand, India

Diploma in Pharmacy from Government Polytechnic, Srinagar Garhwal, Uttarakhand, India

Fellowships & Additional Training

Postdoctoral fellowship: Department of Pharmaceutics, University of Washington (2011-2014)

Teaching

Dr. Prasad is teaching pharmacokinetics principles and PBPK modeling in following courses.

  • PHARDSCI 528
  • PHARMSCI 573

Research

  • Prediction of ontogeny mediated oral absorption and hepatic and renal disposition of drugs in children using physiologically-based pharmacokinetics (PBPK) modeling
  • Discovery and validation of biomarkers for non-invasive and prospective prediction of drug disposition and response
  • Role of drug metabolizing enzymes and transporters in steroid homeostasis

Major expertise: Quantitative proteomics, metabolomics and PBPK modeling

Keywords: Quantitative proteomics, metabolomics, PBPK modeling, pediatric drug disposition, testosterone disposition, non-CYP enzymes

Current funding:

  1. 1R01HD081299-01A1, NIH/NICHD
    PBPK prediction of ontogeny mediated alteration in hepatic drug elimination
    Role: PI
  1. PRINCE (Proteomics-based Research Initiative on Non-CYP enzymes), Multiple Private Sponsors (Genentech, Merck and Gilead)
    Differential tissue abundance of non-CYP enzymes in human and animal models
    Role: PI
  1. R01GM066233, NIGMS/NIH
    The plasma membrane monoamine transporter (PMAT): expression and role in mIBG disposition in neuroblastoma
    Role: Co-investigator (PI: Joanne Wang)
  1. P01GM116691, NIGMS/NIH
    Program on Genetic and Dietary Predictors of Drug Response in Rural and AI/AN Populations
    Role: Co-Investigator (PI: Thummel)
  1. UH3OD023271, NIH
    Prenatal and Early Childhood Pathways to Health
    Role: Co-Investigator (PI: Karr and Sathyanarayana)
  1. mARC proteomics, BMS
    Role of mARC protein in drug metabolism: Interindividual and differential tissue abundance and activity
    Role: PI

Additional information

  • We provide collaborative support to researchers on quantitative proteomics and metabolomics

Professional experience:

  • Assistant Professor (2015-2019), Department of Pharmaceutics, University of Washington, Seattle, WA
  • Director, PRINCE (Proteomics-based Research Initiative on Non-CYP enzymes) (2018-Present), Department of Pharmaceutics, University of Washington, Seattle, WA
  • Co-director, UWRAPT (2016-2019), Department of Pharmaceutics, University of Washington, Seattle, WA
  • Acting Assistant Professor (2014-2015), Department of Pharmaceutics, University of Washington, Seattle, WA
  • Acting Instructor (2012-2014), Department of Pharmaceutics, University of Washington, Seattle, WA
  • Lead Scientist-UWRAPT (2011-2014), Department of Pharmaceutics, University of Washington, Seattle, WA
  • Research Associate (2006-2007), Department of Metabolism and Pharmacokinetics (MAP)-Discovery, Ranbaxy Research Laboratories

Awards and honors:

  • ISSX North American New Investigator Award, International Society for the Study of Xenobiotics (ISSX), ISSX, 2018 Montreal, Canada.
  • Editorial Board Member, Drug Metabolism and Disposition
  • Translational and Clinical Pharmacology (TCP) Division’s Early Career Faculty Showcase ASPET, 2018, San Diego, CA
  • Secretary/Treasurer, Drug Metabolism and Disposition Division, American Society for Pharmacology and Experimental Therapeutics (ASPET)

Selected Publications

  1. Prasad B, Achour B, Artursson P, Hop CECA, Lai Y, Smith PC, Barber J, Wisniewski JR, Spellman D, Uchida Y, Zientek MA, Unadkat JD, Rostami-Hodjegan A. Toward a Consensus on Applying Quantitative Liquid Chromatography-Tandem Mass Spectrometry Proteomics in Translational Pharmacology Research: A White Paper. Clin Pharmacol Ther. 2019 Sep;106(3):525-543.
  2. Ladumor MK, Bhatt DK, Gaedigk A, Sharma S, Thakur A, Pearce RE, Leeder JS, Bolger MB, Singh S, Prasad B. Ontogeny of Hepatic Sulfotransferases and Prediction of Age-Dependent Fractional Contribution of Sulfation in Acetaminophen Metabolism. Drug Metab Dispos. 2019 Aug;47(8):818-831.
  3. Li CY, Basit A, Gupta A, Gáborik Z, Kis E, Prasad B. Major glucuronide metabolites of testosterone are primarily transported by MRP2 and MRP3 in human liver, intestine and kidney. J Steroid Biochem Mol Biol. 2019 Apr 5;.
  4. Bhatt DK, Mehrotra A, Gaedigk A, Chapa R, Basit A, Zhang H, Choudhari P, Boberg M, Pearce RE, Gaedigk R, Broeckel U, Leeder JS, Prasad B. Age- and Genotype-Dependent Variability in the Protein Abundance and Activity of Six Major Uridine Diphosphate-Glucuronosyltransferases in Human Liver. Clin Pharmacol Ther. 2019 Jan;105(1):131-141.
  5. Xu M, Saxena N, Vrana M, Zhang H, Kumar V, Billington S, Khojasteh C, Heyward S, Unadkat JD, Prasad B. Targeted LC-MS/MS Proteomics-Based Strategy To Characterize in Vitro Models Used in Drug Metabolism and Transport Studies. Anal Chem. 2018 Oct 16;90(20):11873-11882.
  6. Zhang H, Basit A, Busch D, Yabut K, Bhatt DK, Drozdzik M, Ostrowski M, Li A, Collins C, Oswald S, Prasad B. Quantitative characterization of UDP-glucuronosyltransferase 2B17 in human liver and intestine and its role in testosterone first-pass metabolism. Biochem Pharmacol. 2018 Oct;156:32-42.
  7. Basit A, Amory JK, Prasad B. Effect of Dose and 5α-Reductase Inhibition on the Circulating Testosterone Metabolite Profile of Men Administered Oral Testosterone. Clin Transl Sci. 2018 Sep;11(5):513-522.
  8. Bhatt DK, Basit A, Zhang H, Gaedigk A, Lee SB, Claw KG, Mehrotra A, Chaudhry AS, Pearce RE, Gaedigk R, Broeckel U, Thornton TA, Nickerson DA, Schuetz EG, Amory JK, Leeder JS, Prasad B. Hepatic Abundance and Activity of Androgen- and Drug-Metabolizing Enzyme UGT2B17 Are Associated with Genotype, Age, and Sex. Drug Metab Dispos. 2018 Jun;46(6):888-896.
  9. Bhatt DK, Prasad B. Critical Issues and Optimized Practices in Quantification of Protein Abundance Level to Determine Interindividual Variability in DMET Proteins by LC-MS/MS Proteomics. Clin Pharmacol Ther. 2018 Apr;103(4):619-630.
  10. Vrana M, Whittington D, Nautiyal V, Prasad B. Database of Optimized Proteomic Quantitative Methods for Human Drug Disposition-Related Proteins for Applications in Physiologically Based Pharmacokinetic Modeling. CPT Pharmacometrics Syst Pharmacol. 2017 Apr;6(4):267-276.
  11. Prasad B, Gaedigk A, Vrana M, Gaedigk R, Leeder JS, Salphati L, Chu X, Xiao G, Hop C, Evers R, Gan L, Unadkat JD. Ontogeny of Hepatic Drug Transporters as Quantified by LC-MS/MS Proteomics. Clin Pharmacol Ther. 2016 Oct;100(4):362-70.
  12. Prasad B, Unadkat JD. Optimized approaches for quantification of drug transporters in tissues and cells by MRM proteomics. AAPS J. 2014 Jul;16(4):634-48.

Bhagwat Prasad’s Bibliography https://www.ncbi.nlm.nih.gov/myncbi/bhagwat.prasad.1/bibliography/public/